Quality Reagents for Your Lab

These high-quality qPCR multiplex reagents are provided to your lab as unpledged assays. All panels are available for purchase before nomination and pledging.

Together with you, LISA will determine which assays will be nominated.

An assay in the table below will be updated when it:

  1. has been nominated and is in the pledge phase,
  2. has launched into a 510(k) or PMA project,
  3. has required a change in formulation after additional validation and verification,
  4. is available in cGMP format
  5. has been included in a pre-submission to the FDA.

Please note that nominated assays may not reach initiation pledge threshold from LISA members.

qPCR Multiplex Panels

All qPCR multiplex panels use common fluorescent channels, are Made in America, and include MasterMix. Four multiplex targets are provided per tube.

Reagents are distributed to LISA members by BlazedX, working in conjunction with the other LISA anchor companies. Through LISA, your lab can also get in-person assistance from BlazedX Field Application Scientists.

qPCR Multiplex Probe + Primer Sets

UT Microbiota Bundles*

qPCR profiling of 12 common urinary tract microbiota

These three UT Microbiota Panels are designed for qualitative multi-plex in-vitro assessment of common urinary tract microbiota, using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. Positive controls included. 

  • 125 reactions with 15ul total volume, or 100 reactions with 20 ul total volume
  • Multiplexes with 4 pathogen targets and one positive control in each tube 

Colors: Detection of Cy5, Cy5.5/HEX, FAM, TexasRED615 and HEX/TAMRA or similar wavelengths

Instruments: BioRad CFX 96 or 384, QuantStudio 5, 6, 7 or 12K

Bacterial-UTI4:
Enterococcus faecalis
Escherichia coli
Klebsiella pneumoniae
Staphylococcus aureus
human RPP30 DNA

Bacterial & yeast-UTI7:
Candida genus
Enterobacter cloacae
Pseudomonas aeruginosa
Staphylococcus epidermidis
human RPP30 DNA

Coming soon:
Enterococcus faecium
Proteus mirabilis
Streptococcus agalactiae (GBS)
Staphylococcus saprophyticus
human RPP30DNA

Bacterial-UTI4:
BUN-UTI4-D-100

Bacterial & yeast-UTI7:
BUN-UTI7-D-100

Coming soon:
TBD

qPCR profiling of 24 common urinary tract microbiota

The Lioness UT Microbiota Panel is designed for qualitative multi-plex in-vitro assessment of common urinary tract microbiota, using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. Positive controls included. 

  • 48
  • 8 multiplexes per reaction, plus 3 positive controls

Detection of FAM, HEX, Cy5 and ROX or similar wavelengths

Acinetobacter baumannii
Klebsiella aerogenes
Enterobacter cloacae
Enterococcus faecium
Escherichia coli
Klebsiella pneumoniae
Staphylococcus aureus
Enterococcus faecalis
Klebsiella oxytoca
Morganella morganii
Proteus mirabilis
Providencia stuartii
Citrobacter freundii
Staphylococcus saprophyticus
Candida glabrata
Candida tropicalis
Candida parapsilosis
Candida albicans
Proteus vulgaris
Pseudomonas aeruginosa
Streptococcus agalactiae (grp B)
Serratia marcescens
Mycoplasma hominis
Ureaplasma urealyticum
human RNaseP (hRPP) (control)

LUTI3.0-PA-48

qPCR profiling of 36 common vaginal microbiota & resistance markers

The Amur WH Vaginal Microbiota Panel is designed for qualitative multi-plex in-vitro assessment of common vaginal microbiota and resistance markers, using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. Positive controls included.

  • 48
  • 10 multiplexes per reaction, plus 4 positive control tubes

Detection of FAM, HEX, Cy5 and ROX or similar wavelengths

HSV 2
HSV1
Treponema pallidum
Gardnerella vaginalis
Neisseria gonorrhoeae
Chlamydia Trachomatis
Trichomonas vaginalis
Haemophilus ducreyi
Mycoplasma genitalium
Ureaplasma urealyticum
Atopobium vaginae
Group B Strep
Escherichia Coli
Enterococcus faecalis
Prevotella bivia
Staphylococcus aureus
Bacteroides fragilis
Mycoplasma hominis
Lactobacillus crispatus
Lactobacillus Jensenii
Lactobacillus Iners
Lactobacillus gasseri
Megasphaera 1
Megasphaera 2
Mobiluncus Curtisii
Mobiluncus mulleris
Candida krusei
Candida Tropicalis
Candida galabrata
Candida parapsilosis
Candida albicans
Candida lusitania
Candida auris
VanA (Antibiotic resistance gene)
MecA (Antibiotic resistance gene)
VanB (Antibiotic resistance gene)
human RNaseP (hRPP) (control)

LWH1.0-PA-48

qPCR profiling of 36 common vaginal microbiota & resistance markers

qPCR profiling of 9 common bacteria, viruses and microorganisms typically associated with transmission by sexual activity

  • 48
  • 3 multiplexes per reaction, plus 2 positive control tubes

Detection of FAM, HEX, Cy5 and ROX or similar wavelengths

Neisseria gonorrhoeae
Chlamydia trachomatis
Trichomonas Vaginalis
HSV 2 (Genital)
HSV 1 (oral)
Treponema pallidum
Ureaplasma urealyticum
Mycoplasma genitalium
Haemophilus ducreyi
Human RnaseP (hRPP) (control)

PSTI1.0-PA048

Detection of 30 bacteria associated with wounds or tissue damage

The Glyphis Wound Panel is designed for qualitative multi-plex in-vitro assessment of bacteria associated with wounds or tissue damage, using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. Positive controls included.

  • 48
  • 9 multiplexes per reaction, plus 4 positive control tubes

Detection of FAM, HEX, Cy5 and ROX or similar wavelengths

Acinetobacter baumannii
Escherichia coli
Enterococcus faecalis
Klebsiella oxytoca
Klebsiella pneumoniae
Enterobacter cloacae
Citrobacter freundii
Proteus mirabilis
Morganella morganii
Enterobacter (Klebsiella) aerogenes
Enterococcus faecium
Streptococcus Pyogenes (Strep A)
Staphylococcus aureus
Streptococcus agalactiae
Staphylococcus saprophyticus
Serratia marcescens
Candida krusei
Candida Tropicalis
Candida galabrata
Candida parapsilosis
Candida albicans
Candida lusitania
Candida auris
Clostridium septicum
Clostridium Novyi
Kingella Kingae
Bacteroides fragilis
Clostridium perfringens
Proteus vulgaris
Pseudomonas aeruginosa
human RNaseP (hRPP) (control)

WPA1.0-PA-48

For qualitative multi-plex in-vitro assessment of viral and bacterial targets

The Amphisense Respiratory Panel is designed for qualitative multi-plex in-vitro assessment of viral and bacterial targets, using nucleic acid amplification. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. Positive controls included.

  • 48
  • 4 multiplexes per reaction, plus 3 positive control tubes

Detection of FAM, HEX, Cy5 and ROX or similar wavelengths

SARS-CoV-2 (durable)
Influenza A (pan influenza)
Influenza B
Rhinovirus
Enterovirus
Enterovirus D68
Adenovirus
Coronavirus 229E
Coronavirus HKU1
Coronavirus OC43
Parainfluenza 1-3
RSV (A & B)
Human metapneumovirus
Klebsiella pneumoniae
Bordetella pertussis
Bordetella parapertussis
Chlamydophila pneumoniae
Mycoplasma pneumoniae
Staphylococcus aureus
Moraxella catarrhalis
Haemophilus influenzae
Streptococcus pneumoniae
Streptococcus pyogenes
Antibiotic resistance MecA
Human RNaseP (hRPP)

ARP1-XL-48

Detection of 21 bacterial antibiotic resistant genes

The Pongo Antibiotic Resistance Panel is designed for qualitative multi-plex in-vitro assessment of antibiotic resistant genes, using nucleic acid amplification. Often used as a reflex assay. Following nucleic acid extraction, the targets are amplified by real-time PCR (qPCR) and detected via fluorescent dye. Positive controls included.

  • 48
  • 3 multiplexes per reaction, plus 3 positive control tubes

Detection of FAM, HEX, Cy5 and ROX or similar wavelengths

ACT
ampC Gene
Fox
mecA
qnrB
qnrA
qnrS vanA1_vanA2
vanB
NDM-1
KPC, bla-KPC
OXA-48, bla-OXA
VIM, bla-VIM
IMP-7, bla-IMP
SHV
TEM
CTX-M group 1
CTX-M Group 2
CTX-M Group 9
ErmA
ErmB
SUL1
SUL2
Tet(A)

PAR2.0-PA-48

* For Research Use Only. Not intended for in-vitro diagnostics. Not approved by the FDA. Distributed by BlazedX, a licensed distributor of PCR Assays & SeqOnce Biosciences products.